EZcodon Instructions
EZcodon processes protein-coding mitochondrial genes to:
- Clean and pad sequences from FASTA files for each strand (Heavy/J or Light/N).
- Concatenate genes across taxa consistently.
-
Compute codon usage metrics:
- Relative Synonymous Codon Usage (RSCU)
- Amino acid frequencies per species
-
Generate plots:
- Line plots (≤20 species) or box plots (>20 species) for amino acid frequencies
- Stacked RSCU plots per strand for each species
Supports analysis for Heavy strand (J), Light strand (N), or both (JN).
- Prepare FASTA files for protein-coding genes for each strand. Each file is named by gene and may contain one or more sequences. Taxa must be named consistently across files.
-
Accepted extensions:
.fasta, .fa, .fsa, .fas, .fna, .ffn, .faa, .frn -
Upload either:
- Only J genes
- Only N genes
- Both J and N genes
- Select the correct mitochondrial genetic code. More info
-
Output includes:
- RSCU CSV tables
- Amino acid frequency CSV tables
- PDF plots
Upload Your Files
*If not provided, a random job ID will be assigned.