EZmito2

EZcodon Instructions

EZcodon processes protein-coding mitochondrial genes to:

Clean and pad sequences from FASTA files for each strand (Heavy/J or Light/N).
Concatenate genes across taxa consistently.
Compute codon usage metrics:
- Relative Synonymous Codon Usage (RSCU)
- Amino acid frequencies per species
Generate plots:
- Line plots (≤20 species) or box plots (>20 species) for amino acid frequencies
- Stacked RSCU plots per strand for each species

Supports analysis for Heavy strand (J), Light strand (N), or both (JN).

Prepare FASTA files for protein-coding genes for each strand. Each file is named by gene and may contain one or more sequences. Taxa must be named consistently across files. Acceptable extensions are: .fasta, .fa, .fsa, .fas, .fna, .ffn, .faa, .frn
Upload your files using the drag & drop zones according to their strands.
Select the correct mitochondrial genetic code for translation. More info.
The output will include CSV tables for RSCU and amino acid frequencies, and PDF plots per strand.

Job Name*

J Genes FASTA Files

Drag & drop J gene FASTA files here
or click to select (multiple allowed)

N Genes FASTA Files

Drag & drop N gene FASTA files here
or click to select (multiple allowed)

Select mitochondrial genetic code

*If not provided, a random job ID will be assigned.