EZmito2 Web Server

EZmito2 is a bioinformatic toolkit for phylogenetic data preparation, nucleotide composition analysis of mitogenomes, detection of chimeric regions produced during the assembly process and other functions.

(version 2026.3, March 2026)

EZmito2's goal is to help users to perform mitogenomic analyses with zero difficulties. Its usefulness stands in the creation of smart mitogenomic data sets for phylogenetic purposes (EZsplit and EZpipe), the calculation/visualization of nucleotide biases (EZskew) as well as codon usage (EZcodon), and the detection of chimeric nucleotide regions (EZmix). Users can also access graphical mitochondrial map representations (EZmap) or re-arrange their genome from a different starting gene (EZcircular). EZdist computes pairwise genetic distances and produces distance matrices and heatmaps, while EZpcoa performs distance-based ordination for multivariate population analyses and EZpopstat provides standard population genetics statistics including pairwise F_ST and hierarchical AMOVA. Further, EZtrampo allows users to partition mitochondrial genome datasets depending on their transmembrane position. EZmito2 is designed to be a fast and solid bioinformatic tool for mitogeneticists and evolutionary biologists. Further information on how these tools work can be found at the corresponding pages.

Some information about ESZ_Lab

The Evolutionary and Systematic Zoology Laboratory, based at the Life Science Department of the University of Siena (Italy), has been working on mitogenomes for over two decades. We have tested different approaches to the study of mitogenomes from a structural standpoint as well as a marker for phylogeny and phylogeography. A simple, though solid, selection of the methods we use in our daily work is now proposed for use in EZmito. Some of our works on mitochondrial DNA and its application can be read here, here or more recently here.

More information about ESZ_lab are available at this link.