EZmito Web Server

EZmito2 is a bioinformatic toolkit for phylogenetic data preparation, nucleotide composition analysis of mitogenomes, detection of chimeric regions produced during the assembly process and other functions.

(version 2026.3, March 2026)

EZmito2's goal is to help users to perform mitogenomic analyses with zero difficulties. Its usefulness stands in the creation of smart mitogenomic data sets for phylogenetic purposes (EZsplit and EZpipe), the calculation/visualization of nucleotide biases (EZskew) as well as codon usage (EZcodon) and the chimerical nucleotide regions detection (EZmix). Users can also access to graphical mitochondrial map representation (EZmap) or re-arrange their genome from a different starting gene (EZcircular). Further, EZtrampo allows users to partition mitochondrial genome dataset depending on their transmembrane position. EZmito2 is designed to be a fast and solid bioinformatic tool for mitogenetists and evolutionary biologists. Further information on how these tools work can be found at the corresponing pages.

Some information about ESZ_Lab

The Evolutionary and Systematic Zoology Laboratory, based at the Life Science Department of the University of Siena (Italy), has been working on mitogenomes for over two decades. We have tested different approaches to the study of mitogenomes from a structural standpoint as well as a marker for phylogeny and phylogeography. A simple, though solid, selection of the methods we use in our daily work is now proposed for use in EZmito. Some of our works on mitochondrial DNA and its application can be read here, here or more recently here.

More information about ESZ_lab are available at this link.