EZmap Instructions


EZmap generates linear or circular graphical maps of mitochondrial genomes using GFF3 or BED annotation files. It highlights genomic features including:

  • Protein-coding genes (CDS)
  • tRNAs and rRNAs
  • A+T rich / control regions

Both formats are accepted — EZmap auto-detects and converts BED to GFF3 internally. Forward strand features are colored using different colors. The output is saved as PDF plots per genome.

  1. Annotation file (GFF3 or BED): EZmap accepts both formats and converts automatically.
    • GFF3 — standard GFF3 with ##gff-version 3 header (.gff, .gff3). Check here for GFF3 format.
    • BED — 6 fields: chrom, chromStart, chromEnd, name, score, strand (.bed, .txt).
  2. Gene names should be short (e.g., cox1) for better visualization.
  3. If genome is incomplete, select linear mode; otherwise circularization is applied automatically.
  4. Upload your annotation file, choose options, and download the results.

Upload Your Files


Drag & drop GFF3 or BED file here
or click to select

*If not provided, a random job ID will be assigned.