EZskew Instructions
EZskew calculates nucleotide biases along protein-coding genes of metazoan mitogenomes, avoiding manual errors. It computes:
- A-T bias
- Strand bias for J (light) and N (heavy) strands
- Codon position bias for 1st, 2nd, and 3rd positions (including 2-fold and 4-fold degenerate codons, if required)
- Prepare separate FASTA files for genes on the J and N strands. Acceptable formats are:
- Name sequences consistently across genes and strands.
- Ensure only standard nucleotides or IUPAC ambiguity codes (IUPAC) are present.
- Gaps inside sequences will be removed automatically.
.fasta, .fa, .fsa, .fas, .fna, .ffn, .faa, .frn
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