EZskew

EZskew helps users in the fast calculation of nucleotide biases along metazoan mitogenomes' PCGs avoiding operators' errors. It calculates:

• The A-T bias;
• The strand bias (both for the J (light) and N (heavy) strand);
• The codon position bias for first, second and third codon positions (including 2-fold and 4-fold degenerate codon positions, if required)

Use it according to the following instructions:

1. Prepare a folder of fasta Protein Coding Genes files for each strand. Each of the two folders will contain the J oriented and the N oriented genes, respectively. Please name the two folders as 'J' and 'N'. Then, compress (.zip, .rar, .tar, .gz, .7z) them as a single archive with the name you desire.
2. Choose the Genetic Code for the analysis (a list of of available codes as well as some additional information can be found here).
3. Upload the file by clicking the Upload link button.
4. The output will generate a .tsv tabular output and .pdf graphic outputs.

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Example of input file preparation



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Download an input example
Download an output example

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Job name*

Select the compressed archive with PCGs (separated by strand) in fasta format
Select the genetic code to use 2. The Vertebrate Mitochondrial Code 4. The Mold, Protozoan, and Coelenterate Mitochondrial Code 5. The Invertebrate Mitochondrial Code 9. The Echinoderm and Flatworm Mitochondrial Code 11. The Bacterial, Archaeal and Plant Plastid Code 13. The Ascidian Mitochondrial Code 14. The Alternative Flatworm Mitochondrial Code 21. Trematode Mitochondrial Code 24. Rhabdopleuridae Mitochondrial Code 33. Cephalodiscidae Mitochondrial UAA-Tyr Code
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*Useful to organize your processed jobs. If not provided, a random ID will be created.