EZmix Instructions
EZmix analyzes candidate mitochondrial genome sequences to:
- Check sequences for duplicates and gaps.
- Compare all sequences pairwise to identify regions of inter-molecular similarity.
- Filter similar regions based on user-defined thresholds:
- Minimum identity (%)
- Minimum length (bp)
- Generate graphical outputs:
- Linear plots of sequences with similarity regions highlighted
- Color-coding according to identity percentage
- Supports analysis of complete candidate mitochondrial genomes, enabling detection of potential chimeric assemblies or regions of phylogenetic similarity.
- Prepare a FASTA file containing all candidate mitochondrial genomes. Acceptable extensions:
.fasta, .fa, .fsa, .fas, .fna, .ffn, .faa, .frn - Ensure no duplicate sequence IDs and no GAPs in the sequences.
- Sequence names should be informative; graphical output truncates names to 10 characters.
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