EZcircular Instructions
EZcircular takes a mitochondrial genome annotation file (BED or GFF3) and a reference FASTA sequence to:
- Auto-detect the annotation format (BED or GFF3) and convert if needed.
- Validate and parse the annotation.
- Append 100 Ns and an artificial feature if the genome is linear.
- Locate the starting gene and reorder the FASTA sequence.
- Generate an updated BED file with correct coordinates.
- Produce circular or linear genome maps based on the topology.
- Annotation file (BED or GFF3): EZcircular accepts both formats and converts automatically.
- BED — 6 fields:
chrom, chromStart, chromEnd, name, score, strand(.bed, .txt) - GFF3 — standard GFF3 with
##gff-version 3header (.gff, .gff3). Check here for GFF3 format.
- BED — 6 fields:
- FASTA file: Containing only the reference genome sequence (
.fasta, .fa, .fsa, .fas, .fna, .ffn, .faa, .frn). - If the genome is incomplete, select linear topology.
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