EZcircular Instructions
EZcircular takes a mitochondrial genome BED file and a reference FASTA sequence to:
- Validate and parse the BED annotation.
- Append 100 Ns and an artificial BED feature if the genome is linear.
- Locate the starting gene and reorder the FASTA sequence.
- Generate an updated BED file with correct coordinates.
- Produce circular or linear genome maps based on the topology.
Output example: Download
- BED file: Describe your mitochondrial genome annotation with exactly 6 fields:
chrom, chromStart, chromEnd, name, score, strand. Must be in BED format (.bed, .txt), GFF not supported. Convert with gff2bed if needed. - FASTA file: Containing only the reference genome sequence (
.fasta, .fa, .fsa, .fas, .fna, .ffn, .faa, .frn). - If the genome is incomplete, select linear topology.
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