ESZlab1

EZmito Web Server

powered by ESZ_lab (University of Siena)

EZmito is a bioinformatic tool for phylogenetic data preparation, nucleotide composition analysis of mitogenomes and chimerical regions detection produced during the assembly step

(version 2023.12, December 2023)

EZmito is composed of five main tools.

  • Ezsplit helps users in the fast creation of a data set from multiple mitogenomes downloaded from Genbank (NCBI). It was developed to ease users in the correct data submission to EZpipe. It splits mitochondrial gene features in different fasta files (one for each mitochondrial Protein Coding Gene)
  • EZpipe helps users in the fast creation of a data set of aligned and concatenated genetic sequences avoiding operators' errors. It was developed for large data sets (such as phylomitogenomic analyses - diffcult to handle manually), but it can be useful for small matrices as well.
  • EZskew helps users to visualize the most important skew characteristics of mitogenomes. It can calculate the AT-bias, the strand bias and the codon position bias with smart graphical outputs.
  • EZcodon helps users to visualize the codon usage of mitogenomes. It produces a table of codon usage as well as some smart graphical outputs.
  • EZmix helps users to recognize areas of inter molecular similarity that may be indicative of the assembly of chimeric mitochondrial genomes. The tool produces a graphical output where such regions are highlighted.
  • EZmap helps users to create a graphical map of linear and cicular mitogenomes.
  • EZcircle helps users to rea-rrange the order of the mitochondrial genome setting a new starting gene

Issues

Due to its recent launch, EZmito could show little problems in input file parsing and processing. Fixing and updates of the programs are scheduled but errors might be found by users. In this case, please carefully check the input file required for each tool and try again. If the problem still persists do not hesitate to contact us.

Credits

While using EZmito you are actually using third party softwares that are to be acknowledged in any forthcoming publication alongside EZmito.

Contacts

If you want to get in contact with us please mail Claudio Cucini at claudio.cucini2@unisi.it or visit our website.

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